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Chapters
- 00:05Title
- 00:45Whole-cell Biocatalysts Preparation
- 02:05Biosynthesis of L-xylulose by Coupling E. coliHjLAD and E. coliSpNOx for Cofactor Regeneration
- 03:51Immobilization of Recombinant Whole-cell Catalysts in Calcium Alginate Beads
- 06:25Stability Assay of Immobilized Biocatalysts for L-xylulose Production
- 07:15Results: Co-immobilization of Whole-cell Biocatalysts Improves Reusability
- 08:55Conclusion
Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.
