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Een hele berg<em> In Situ</em> Hybridisatie Methode voor de slak,<em> Poelslak</em
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A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
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Een hele berg<em> In Situ</em> Hybridisatie Methode voor de slak,<em> Poelslak</em

DOI:
10.3791/53968-v

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07:33 min

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March 15, 2016

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, ,
1Department of Geobiology,Georg-August University of Göttingen

Chapters

  • 00:05Title
  • 00:52Assembly of Decapsulation Apparatus
  • 01:43Sample Collection, Fixation and Decapsulation
  • 05:48Results: Representative Images of WMISH Expression Patterns Against a Variety of Genes from a Range of L. stagnalis Developmental Stages
  • 06:42Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to provide users with a set of methods for the high-throughput decapsulation of Lymnaea stagnalis embryos and larvae in preparation for whole mount in situ hybridization, and for subsequent pre- and post-hybridization treatments.

Tags

Whole Mount In Situ HybridizationGastropodLymnaea StagnalisEmbryonicLarvalEgg CapsulesSpatial Gene Expression발생학Evo-devoDecapsulationDevelopmental StagingSample Preparation

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