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Cycloheximid Chase-Analyse von Proteinabbau in<em> Saccharomyces cerevisiae</em
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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
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Cycloheximid Chase-Analyse von Proteinabbau in<em> Saccharomyces cerevisiae</em

DOI:
10.3791/53975-v

•

09:05 min

•

April 18, 2016

•

, , ,
1Department of Biology,Ball State University, 2Bioproduct Research & Development,Eli Lilly and Company

Chapters

  • 00:05Title
  • 00:36Growth and Harvest of Yeast Cells
  • 03:14Cycloheximide Chase
  • 05:23Post-alkaline Protein Extraction
  • 06:51Results: Cue1 Is Required for Degradation of Deg1-Sec62
  • 08:23Conclusion

Summary

자동 번역

자동 번역

Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).

Tags

Protein DegradationSaccharomyces CerevisiaeCycloheximide ChaseEndogenous ProteinPlasmid-expressed ProteinOptical DensityMid-logarithmic GrowthHeat BlockStop MixCell LysisProtein Denaturation

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