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Chapters
- 00:05Title
- 01:00Lens Dissection
- 02:24Stiffness Measurements
- 04:51Lens Nucleus Measurement and Image Analysis
- 05:51Results: Axial and Equatorial Strain-load Curves and Morphological Characteristics of 2,4, and 8-month-old WT Mouse Lenses
- 07:05Conclusion
Age-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger animals.
