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En guide til Strukturert Illumination TIRF Mikros i høy hastighet med flere farger
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
DOI:

11:15 min

May 30, 2016

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Chapters

  • 00:05Title
  • 00:54Arranging and Aligning the Exitation Path
  • 04:01Alignment of Polarization Rotator and Emission Path
  • 06:42System Synchronization and Calibration
  • 08:23Results: Multi-colored, High Speed TIRF Microscopy
  • 10:11Conclusion

Summary

자동 번역

This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.

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