JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:52Generation of Marked Synechocytis and Synechococcus Mutants
- 05:16Generation of Unmarked Synechocytis Mutants
- 07:59Long-term Storage of Strains
- 09:15Results: Cyanobacteria Marked and Markerles Species
- 10:52Conclusion
Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.
