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Chapters
- 00:05Title
- 00:48Hydrogel Infiltration
- 01:32Polymerization
- 03:03Lipid Removal
- 03:57Molecular Staining
- 04:32Refractive Index Matching
- 06:36Imaging the Cleared Sample
- 08:43Results: THY1-YFP Expression in the Brain and Spinal Cord
- 09:44Conclusion
Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive results.
