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表达和疫苗病毒样颗粒的纯化

JoVE 신문
생물학

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JoVE 신문 생물학

Expression and Purification of Virus-like Particles for Vaccination

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表达和疫苗病毒样颗粒的纯化

DOI:

10.3791/54041-v

•

06:17 min

•

June 02, 2016

•

Maria T. Arevalo, Terianne M. Wong, Ted M. Ross

¹Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine,University of Georgia

Chapters

00:05Title
00:58Influenza H3N2 Virus-like Particle (VLP) Generation
02:10CHIK VLP Expression Using Baculovirus/Insect Cell System
03:18Sedimentation and Purification of VLPs and SVPs
04:42Results: Successful Expression and Purification of VLPs
05:23Conclusion

Summary

자동 번역

这里，我们提出用于合成使用任一杆状病毒或哺乳动物表达系统，并超速离心纯化病毒样颗粒的协议。这种高度可定制的方式被用来识别在一个安全的，灵活的方式的病毒抗原作为疫苗的目标。

Tags

Virus-like Particles VLPs Vaccination Protein Expression Purification Mammalian Cells Insect Cells Transfection Baculovirus CHIKV Antigen Vaccine Serology Diagnosis

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