This content is Free Access.
Chapters
- 00:05Title
- 00:58Polymer Solution Preparation
- 01:48Agarose and Polymer Film Preparation
- 03:28Formation of Polymersomes
- 04:14Imaging of Polymersomes by Fluorescence Recovery after Photobleaching (FRAP)
- 05:18Characterization of Polymersome Size
- 06:27Results: Imaging and Characterization of Polymersomes
- 07:22Conclusion
We present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies.
