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显微注射转基因的基因组，并在编辑Threespine刺鱼
JoVE 신문
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JoVE 신문 발생학
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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显微注射转基因的基因组，并在编辑Threespine刺鱼

DOI:
10.3791/54055-v

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08:51 min

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May 13, 2016

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, ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Chapters

  • 00:05Title
  • 00:38Prepare for Microinjection
  • 03:21Microinjection and Post Injection Care
  • 06:03Results: Microinjection and Expression of Reporter Constructs in Sticklebacks
  • 07:53Conclusion

Summary

자동 번역

자동 번역

转基因操作和基因组编辑是功能测试基因和顺 -regulatory元素的作用是至关重要的。此处的基因组修饰（包括TOL2介导的荧光报道基因构建体，TALENS和CRISPRs）的生成的详细显微注射协议提出了紧急模型鱼的三刺。

Tags

Keywords: MicroinjectionTransgenesisGenome EditingThreespine SticklebackDevelopmental GeneticsEvolutionary GeneticsGene FunctionEnhancer FunctionBody PatternTransgenic FishFunctional Genetic AnalysisBorosilicate GlassMicropipette NeedlesFertilized EggsInjection SolutionTransilluminationDissecting MicroscopePlaster Saw BladePressure ControlNeedle HolderMicromanipulatorWatchmaker’s ForcepsStickleback Water

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