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Chapters
- 00:05Title
- 01:22Metagenomic Library False Positive Removal
- 03:36Metagenomic Enzyme Screening
- 05:48Results: Representative Enzyme Activity Evaluation
- 06:56Conclusion
This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
Tags
Multi-enzyme ScreeningHigh-throughput Genetic Enzyme Screening SystemMetagenomic ScreeningEnzyme EngineeringMPL MutantPhenylene CompoundGFP ReporterEnzyme ActivityFluorescence MeterMetagenomic LibraryFosmid VectorFosmid Library Production KitPGESS(E135K) DNAFalse PositivesLysogenate BrothAmpicillinChloramphenicolFACS MachinePBSLog-scaled Forward Scatter AreaLog-scaled Side Scattered AreaCell CountLog-scaled FITC-area Histogram
