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A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side
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A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side

A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side

DOI:
10.3791/54061-v

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09:58 min

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May 06, 2016

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, , , , , ,
1Department of Pediatrics, Medical Faculty Mannheim,Heidelberg University, 2Department of NDU Life Sciences,Nippon Dental University

Chapters

  • 00:05Title
  • 01:19Human Choroid Plexus Papilloma (HIBCPP) Cell Culture Filter Insert Seeding
  • 04:25Transepithelial Electrical Resistance (TEER) Measurement
  • 05:50Paracellular Permeability Determination
  • 07:06Results: Representative HIBCPP Barrier Function Analysis
  • 08:48Conclusion

Summary

자동 번역

자동 번역

The epithelial cells of the choroid plexus (CP) form the blood-cerebrospinal fluid barrier (BCSFB). An in vitro model of the BCSFB employs human choroid plexus papilloma (HIBCPP) cells. This article describes culturing and basolateral infection of HIBCPP cells using a cell culture filter insert system.

Tags

Keywords: Choroid Plexus Epithelial CellsBlood-cerebrospinal Fluid BarrierBacterial InfectionBasolateral SideCentral Nervous SystemHIBCPP CellsCell CultureFilter InsertsTranswell SystemTripsyn EDTACell SuspensionCell Culture Method

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