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Immunology and Infection

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JoVE Journal Immunology and Infection
Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
 

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Article DOI: 10.3791/54062-v • 08:02 min • June 27th, 2016
June 27th, 2016 •
1, 2, 1, 1, 1, 1, 1,3, 2, 1,3
1Institute of Cellular Therapeutics, Allegheny Health Network, 2Division of Pharmaceutical Sciences, Mylan School of Pharmacy, Duquesne University, 3Department of Biological Sciences, Carnegie Mellon University

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요약
Automatic Translation

This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.

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Keywords: 3-D Aggregation Thymic Epithelial Cells FACS Purification EAK 16-II/EAKIIH6 Self-assembling Hydrogel Thymus Bioengineering Cell Harvesting Tissue Dissection Enzymatic Digestion Cell Washing Cell Suspension
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