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设计和适体 - 金纳米粒子基于比色法的开发在这领域的应用

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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

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设计和适体 - 金纳米粒子基于比色法的开发在这领域的应用

DOI:

10.3791/54063-v

•

08:23 min

•

June 23, 2016

•

Joshua E. Smith, Jorge L. Chávez³, Joshua A. Hagen, Nancy Kelley-Loughnane

¹Department of Science and Mathematics,Alvernia University, ²711^th Human Performance Wing, Air Force Research Laboratory,Wright-Patterson Air Force Base, ³UES, Inc.

Chapters

00:05Title
00:47Synthesis via Citrate Reduction of Gold Nanoparticles (AuNP) and Characterization
02:35DNA-aptamer, Buffer, Solution, and Assay Preparation
03:35Salt Titration and Assay Setup
05:21Freezing Aptamer–AuNP Assay for Long-term Storage
06:38Results: Assay Response with Changing DNA Coverage Density and Performance of the Assay for Long-term Storage
07:37Conclusion

Summary

자동 번역

进行了检查适体，纳米金比色法检测小分子在得到了实地应用的设计与开发。此外，一个智能设备色度应用（应用程序）的验证，并建立了在该领域使用的测定法的长期贮存。

Tags

Aptamer Gold Nanoparticle Colorimetric Assay In-field Application Reproducibility Shelf-life False Positive Gold Chloride Sodium Citrate DEPC Autoclave Cocaine-binding Aptamer Phosphoramidite Chemistry Oligonucleotide

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