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Chapters
- 00:05Title
- 01:12Cryo-fixation of Leaf Tissues by High-pressure Freezing
- 03:43Freeze Fracture and Double-layer Coating
- 07:10Cryo-scanning Electron Microscopy
- 10:52Results: Cryo-scanning Electron Microscopy Analysis of Photosynthetic Membranes in Freeze-fractured Leaf Tissues
- 12:57Conclusion
Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.
