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Chapters
- 00:05Title
- 00:52DNA and RNA Extraction from Marine Coastal Sediments
- 03:23Preparation of RNA and Quality Check of DNA-free RNA
- 05:30Real-time PCR
- 08:21Results: Quantification of 16S rRNA Genes and Transcripts by Simultaneous DNA-RNA Extraction and Real-time PCR
- 10:35Conclusion
A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.
