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Chapters
- 00:05Title
- 01:39Collecting Bone Marrow from Minipigs
- 02:47Isolation of Cells and In Vitro Cultivation
- 04:42In Vitro Cultivation of Cells Isolated from Bone Marrow
- 06:18Collecting Synovial Fluid and Cultivating Isolated Cells
- 08:19Results: Bone Marrow- and Synovial Fluid-derived MSC Morphology and Cell Surface Marker Expression
- 09:23Conclusion
A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.
