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Chapters
- 00:05Title
- 01:08Hybridization
- 03:02Target Capture
- 06:56Post Capture PCR Amplification
- 09:45Results: The Capture Method Has Multiple Advantages over Non-targeted RNAseq
- 11:13Conclusion
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
Tags
Keywords:
Targeted RNA SequencingGene ExpressionGenomic AlterationsRibosomal RNA DepletionChemical FragmentationCDNA SynthesisBarcoded LibrariesHybridizationTarget CaptureMultiplexed SequencingGene FusionsSingle Nucleotide VariationsAlternative SplicingCustom ProbesCot-1 DNABlocking OligosHybridization BufferStreptavidin Beads
