JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:56Preparation of Nuclear Lysate from Cultured Cells
- 03:19Electrophoretic Mobility Shift Assay (EMSA)
- 06:56DNA Affinity Purification Assay (DAPA)
- 09:37Results: Non-coding Genetic Variants Affect Transcription Factor DNA Binding
- 11:04Conclusion
We present a strategic plan and protocol for identifying non-coding genetic variants affecting transcription factor (TF) DNA binding. A detailed experimental protocol is provided for electrophoretic mobility shift assay (EMSA) and DNA affinity precipitation assay (DAPA) analysis of genotype-dependent TF DNA binding.
