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人类多能干细胞的模板微图案用于探测分化命运的空间组织

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Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

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人类多能干细胞的模板微图案用于探测分化命运的空间组织

DOI:

10.3791/54097-v

•

08:07 min

•

June 17, 2016

•

Geetika Sahni*¹, Jun Yuan*¹, Yi-Chin Toh²

¹Department of Biomedical Engineering,National University of Singapore, ²Singapore Institute of Neurotechnology (SINAPSE),National University of Singapore

Chapters

00:05Title
01:02Preparing the Stencil for Cell Seeding
02:32Seeding Human Embryonic Stem Cells onto the Stenciled Substrate
04:39Stencil Removal and Passivation of Unpatterned Substrate
06:20Results: Mechanically Patterning Early Mesoendermal Differentiation
07:39Conclusion

Summary

자동 번역

人多能干细胞（hPSCs）具有分化和自我组织成不同的组织型态的内在能力;尽管这需要空间环境梯度的表现。我们提出模版图案化作为一种简单而可靠的方法，以产生生物化学和机械梯度用于控制HPSC分化模式。

Tags

Stencil Micropatterning Human Pluripotent Stem Cells Spatial Organization Stem Cell Differentiation Cell-cell Adhesion Cell-matrix Adhesion PDMS Stencil Basement Membrane Matrix Plasma Treatment HESC Colonies

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