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पर्यावरण के नमूनों से नई Faustoviruses के अलगाव का उपयोग के लिए एक तेजी रणनीति<em> Vermamoeba vermiformis</em
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
DOI:

09:27 min

June 04, 2016

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Chapters

  • 00:05Title
  • 00:52Host Preparation for Blind Culture Procedures and Sample Inoculation
  • 02:59Detection of Lysis by Automated Flow Cytometry
  • 04:51Preliminary Staining to Reveal the Presence of Giant Viruses and Electron Microscopy
  • 06:59Results: Detection of Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
  • 07:55Conclusion

Summary

자동 번역

We describe here the latest advances in viral isolation for the characterization of new genotypes of Faustovirus, a new asfarvirus-related lineage of giant viruses. This protocol can be applied to the high throughput isolation of viruses, especially giant viruses infecting amoeba.

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