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Chapters
- 00:05Title
- 04:50Preparing the Caspase Tracker Biosensor Flies
- 09:29Transient Cell Death Induction
- 11:10Fixation and Staining of Egg Chambers
- 13:58Results: Reversal of Cell Death Process in Drosophila
- 18:44Conclusion
Anastasis is technically challenging to detect in vivo because the cells that have reversed the cell death process can be morphologically indistinguishable from normal healthy cells. Here we describe protocols for detecting and tracking cells that undergo anastasis in live animals by using our newly developed in vivo CaspaseTracker biosensor system.
