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Chapters
- 00:05Title
- 00:39Grafting Amine Functional Groups on Cellulose Paper Discs
- 03:10Paper-based ELISA for IgG Detection
- 06:28Results: Analysis of the Binding Activity of Immobilized Antibodies to the Covalent Immobilized Cellulose Paper Matrix
- 08:21Conclusion
In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.
