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Chapters
- 00:05Title
- 00:34mRNA Transcription of Transcription Activator-like Effector Nucleases
- 02:18Inject Astyanax mexicanus Embryos with TALEN mRNA
- 04:06Phenotype Founder Fish and Evaluate TALEN Efficiency
- 05:27Results: Germline Transmission of TALEN-induced oca2 Mutations in A. mexicanus
- 06:47Conclusion
Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.
