/

/

为患者组织甲基结合DNA序列捕获

JoVE 신문
유전학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 유전학

Methyl-binding DNA capture Sequencing for Patient Tissues

Please note that all translations are automatically generated. Click here for the English version.

为患者组织甲基结合DNA序列捕获

DOI:

10.3791/54131-v

•

08:40 min

•

October 31, 2016

•

Rohit R. Jadhav, Yao V. Wang, Ya-Ting Hsu, Joseph Liu, Dawn Garcia, Zhao Lai, Tim H. M. Huang, Victor X. Jin

¹Department of Molecular Medicine,University of Texas Health Science Center at San Antonio, ²Greehey Children’s Cancer Research Institute,University of Texas Health Science Center at San Antonio

Chapters

00:05Title
01:18Methyl-binding DNA Capture
02:10Single Fraction Elution
03:08Ethanol Precipitation
04:29Sequencing
05:58Results: Sequencing of Methyl-binding DNA Capture
07:15Conclusion

Summary

자동 번역

在这里，我们提出了一个协议，调查使用甲基结合DNA测序捕获（MBDCap-SEQ或MBD-SEQ）技术以及随后的生物信息学分析管道的大规模临床病人的筛查研究全基因组DNA甲基化。

Tags

DNA Methylation Epigenetics Patient Cohorts Biomarkers Methyl-binding DNA Capture Sequencing Genome-wide Analysis Cost-effective Sonication Fragment Size Elution Magnetic Beads

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

超高分子量基因组DNA的分离纯化白粉病对长读测序

适用于下一代测序的植物细菌DNA富集方法的优化与比较分析

在慢性淋巴细胞性白血病患者中使用甲基CpG结合域捕获法进行综合DNA甲基化分析

植物标本标本的鲁棒 DNA 分离与高通量测序库建设

大范围早期果蝇胚胎的全基因组染色质构象捕获库的生成

回顾性 MicroRNA 排序: 用福尔马林固定石蜡嵌入 RNA 标本补充 DNA 文库制备协议

基于纠错 DNA 和 RNA 测序的稀有事件检测方法

利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列

用于抗倍体植入前基因测试的半导体测序

纳米测序全血 mRNA 测序

Read Article