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高度敏感和快速荧光检测用便携式分析仪FRET

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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

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高度敏感和快速荧光检测用便携式分析仪FRET

DOI:

10.3791/54144-v

•

08:27 min

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October 01, 2016

•

Haseong Kim*¹, Gui Hwan Han*¹, Yaoyao Fu, Jongsik Gam, Seung Goo Lee³

¹Synthetic Biology & Bioengineering Research Center,Korea Research Institute of Bioscience and Biotechnology, ²College of Interdisciplinary & Creative Studies,Konyang University, ³Biosystems and Bioengineering Program,University of Science and Technology

Chapters

00:05Title
00:48Förster Resonance Energy Transfer (FRET) Biosensor Preparation
04:25Analysis of Sugar Content of Beverages Using a FRET Biosensor
06:31Results: Quantitative Analysis of Sugar Content Using a Protein Biosensor for Portable FRET Analyzer
07:49Conclusion

Summary

자동 번역

这个协议描述了使用定制便携式FRET分析器荧光共振能量转移（FRET）的传感器数据的快速且高灵敏度定量。该装置被用于最大化的检测灵敏度，有利的糖含量的实际和有效的评估的临界温度范围内检测麦芽糖。

Tags

Fluorescence Detection FRET Analyzer Sugar Content Small Molecule Sensing Biosensor E. Coli Plasmid Electroporation LB Agar Seed Culture IPTG

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