JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 01:04Preparation of the Silica Colloidal Particle Suspensions
- 04:38Fabrication of the PDMS Microfluidic Chip
- 08:14Experiment for Electrokinetic Concentration of DNA
- 10:12Conclusions
We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.
Tags
Nanofluidic JunctionPDMS Microfluidic ChipSelf-assemblyColloidal ParticlesSilica NanoparticlesCation-selective MembraneDNA And Protein ConcentrationDouble-molding ProcessSurface FunctionalizationIon DepletionConcentration IncreaseSilica Bead SuspensionPolyelectrolytes (PAHPSS)Surface Charge Modification
