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Chapters
- 00:05Title
- 00:59Window Chamber Installation
- 03:04Combined Multi-photon and Confocal Microscopy Imaging
- 05:31Image Analysis and Quantification
- 06:52Results: Representative Neutrophil Vasculature Infiltration Tracking
- 07:58Conclusion
The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.
