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Analyse de Brain mitochondries utilisant Serial Bloc-Face microscopie électronique à balayage
JoVE 신문
신경과학
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JoVE 신문 신경과학
Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
DOI:

07:47 min

July 09, 2016

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Chapters

  • 00:05Title
  • 00:54Brain Tissue Preparation
  • 03:32SBFSEM Imaging
  • 04:15Analyzing the Imaging Dataset
  • 05:43Results: Analysis of Brain Mitochondrial Morphology, Distribution, Abundance and Ultrastructure Using Serial Block-Face Scanning Electron Microscopy
  • 07:04Conclusion

Summary

자동 번역

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.

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