/

/

饲养果蝇<em>果蝇</em>在无菌和悉生条件

JoVE 신문
발생학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Please note that all translations are automatically generated. Click here for the English version.

饲养果蝇<em>果蝇</em>在无菌和悉生条件

DOI:

10.3791/54219-v

•

09:34 min

•

July 30, 2016

•

Melinda L. Koyle, Madeline Veloz, Alec M. Judd, Adam C.-N. Wong⁴, Peter D. Newell⁵, Angela E. Douglas³, John M. Chaston²

¹Department of Plant and Wildlife Sciences,Brigham Young University, ²Department of Entomology,Cornell University, ³Department of Molecular Biology and Genetics,Cornell University, ⁴Division of Infectious Diseases, Boston Children’s Hospital,Harvard Medical School, ⁵Biological Sciences,SUNY Oswego

Chapters

00:05Title
00:43Bacterial Culture and Diet Preparation
02:16Egg-laying Cages
03:13Egg Collection
03:55Egg Dechorionation and Sterile Diet Transfer
05:33Gnotobiotic Fly Generation
06:40Measuring CFU Load and Testing for Sterility
08:05Results: Successful Axenic Fly Rearing Confirmed with CFUs
08:49Conclusion

Summary

자동 번역

提出了一种用于无菌和限菌条件下饲养果蝇的方法。飞胚dechorionated在次氯酸钠，无菌转移到无菌的饮食，并在密闭容器中饲养。接种饮食和胚胎与细菌导致限菌协会，和细菌的存在是通过电镀全身果蝇匀浆证实。

Tags

Drosophila Melanogaster Axenic Gnotobiotic Microbiota Sterile Diet MRS Agar Lactobacillus Acetobacter Grape Juice Agar Yeast Paste Egg Collection

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

的测定血细胞种群<em>果蝇</em>幼虫

生产和治疗间充质干行政/基质细胞（MSC）球体底漆在三维培养下无异物条件

协议的可视化类固醇机关及其互动器官在果蝇免疫染色<em>果蝇</em

成人果蝇脑内蘑菇体和感光神经元的解剖和荧光染色

低血清条件下心脏祖细胞内皮分化的诱导作用

解冻、培养和冷冻保存的嗜酸性粒细胞系

检测果蝇黑色素的内分泌干扰的方法

果蝇黑色素血脑屏障完整性分析

在无馈体和化学定义的培养条件下，使用人类多能干细胞的后多能感感神经元的有效分化

使用微计算断层扫描的果蝇黑色素加斯特全动物成像

Read Article