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Immunology and Infection

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JoVE Journal Immunology and Infection
Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
 

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

Article DOI: 10.3791/54237-v • 07:04 min • July 21st, 2016
July 21st, 2016 •
1,2, 3, 3, 1,4,5
1Benioff Children’s Hospital, 2Department of Pathology, University of California, San Francisco, 3Center for Reproductive Sciences and Department of Obstetrics, University of California, San Francisco, 4Program in Microbial Pathogenesis and Host Defense, University of California, San Francisco, 5Biomedical Sciences Program, University of California, San Francisco

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요약
Automatic Translation

A simple method to establish primary human placental (villous) and decidual organ cultures is described. Villous and decidual organ cultures are invaluable tools for studying pathogenesis at the human maternal-fetal interface. Infection with the facultative intracellular bacterium Listeria monocytogenes is demonstrated.

Tags

Human Placental Cultures Decidual Organ Cultures Maternal-fetal Interface Infections Placental Biology Infectious Diseases Three Dimensional Tissue Structure Fresh Human Specimens Tissue Culture Technique Extra-cellular Matrix Vials Collection Medium Trans-well Inserts Six Well Tissue Culture Plate
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