Journal
/
생물학
/
Gennemførelse af en sammenhængende Anti-Stokes Raman Scattering (CARS) System på en Ti: Sapphire og OPO laserbaseret Standard Laser Scanning Microscope
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Gennemførelse af en sammenhængende Anti-Stokes Raman Scattering (CARS) System på en Ti: Sapphire og OPO laserbaseret Standard Laser Scanning Microscope

DOI:
10.3791/54262-v

•

12:54 min

•

July 17, 2016

•

, , , ,
1INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier, 2Université de Nîmes, 3CNRS, IES, UMR 5214, 4Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249, 5Montpellier RIO Imaging (MRI)

Chapters

  • 00:05Title
  • 00:57Laser System Startup and Microscope Setup
  • 02:13Temporal Synchronization: Part 1
  • 04:25Temporal Synchronization: Part 2
  • 06:46Spatial Overlap of the Beams
  • 08:42Final Adjustments for CARS Signal Observation
  • 10:32Results: Myelin Sheath and Collagen Fibers Viewed by CARS
  • 12:05Conclusion

Summary

자동 번역

자동 번역

Sammenhængende anti-Stokes Raman spredning (CARS) mikroskopi baseret på iboende vibration af molekyle obligationer tillader label-fri kemisk selektiv levende celler. Dette arbejde viser gennemførelsen af ​​en supplerende mikroskopi teknik på en standard multifoton laser scanning mikroskop baseret på et femtosekund Ti: safir laser og en OPO laser.

Tags

Coherent Anti-Stokes Raman Scattering (CARS)Ti:Sapphire LaserOPO LaserLaser Scanning MicroscopyLabel-free ImagingChemically Selective ImagingLipid-related DiseasesSkin PenetrationSkin DisordersDichroic MirrorNarrow Band Pass FilterPMTFluorescenceSHGDelay LinePhoto DiodeOscilloscope

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/54262?article_type=v

Laser Capture Microdissection of Mammalian Tissue
check_url/kr/54262?article_type=v

Laser-Induced Chronic Ocular Hypertension Model on SD Rats
check_url/kr/54262?article_type=v

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
check_url/kr/54262?article_type=v

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
check_url/kr/54262?article_type=v

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
check_url/kr/54262?article_type=v

Dithranol som en matrix til Matrix Assisted Laser Desorption / Ionisering Imaging på en Fourier Transform-cyklotron resonans Mass Spectrometer
check_url/kr/54262?article_type=v

Real Time In Vivo Tracking af thymocytter i det forreste kammer i øjet ved Laser Scanning mikroskopi
check_url/kr/54262?article_type=v

Label-Free Imaging af Lipid Storage Dynamics i Caenorhabditis elegans ved hjælp af stimuleret Raman Scattering Mikroskopi
check_url/kr/54262?article_type=v

Konfokal laserscanning Mikroskopi af calciumdynamik i akutte musespytkirtelvævsskiver
check_url/kr/54262?article_type=v

Hurtig antimikrobiel følsomhedstest ved stimuleret Raman-spredningsbilleddannelse af deuteriuminkorporering i en enkelt bakterie

Read Article