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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

DOI:
10.3791/54277-v

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08:57 min

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July 25, 2016

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,
1Department of Developmental Pathology,Institute of Pathology, University of Bonn Medical School

Chapters

  • 00:05Title
  • 00:40293T Cell Lentiviral Vector Production
  • 03:06Fibroblast to Induced Trophoblast Stem Cell (iTSC) Conversion
  • 05:10Individual iTSC Line Isolation (Transdifferentiation Day 21-28)
  • 07:02Results: Representative Morphological Changes During MEF to iTSC Conversion
  • 07:56Conclusion

Summary

자동 번역

자동 번역

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

Tags

Murine Embryonic FibroblastsTrophoblast Stem CellsInduced Trophoblast Stem CellsITSCsTransfectionLentiviral Vector293T CellsVirus ProductionCell CulturePoly-L-lysineChloroquineHEPES Buffered Saline

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