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Chapters
- 00:05Title
- 01:30Establishing the Blood-brain Barrier Model
- 05:24Blood-brain Barrier Validation: TEER Measurement and Trans-BBB Flux of FD40
- 06:20FD40 Flux and Trans-BBB Permeation of FTIC-loaded Ferritins (FnN)
- 08:21Results: Trans-BBB Flux of Nanoformulated FITC and Confocal Microscopy of RBMECs on Inserts
- 09:58Conclusion
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
