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Chapters
- 00:05Title
- 01:10siRNA Transfection Assay
- 02:30Western Blot Assay for siRNA-transfected Cells
- 04:39Evaluation of Cell Migration with Scratch-induced Directional Wounding Assay
- 05:55Results: Western Blot Analysis and Scratch-induced Directional Wounding Assay to Evaluate Cell Migration
- 07:21Conclusion
Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.
