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Bereiding van formaline gefixeerde paraffine-ingebed weefsel Cores voor zowel RNA en DNA-extractie
JoVE 신문
생물학
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JoVE 신문 생물학
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
DOI:

08:30 min

August 21, 2016

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Chapters

  • 00:05Title
  • 00:57Tissue Coring
  • 02:40Deparaffinization of the FFPE Tissue Cores
  • 03:45Homogenization of the Deparaffinized Cores
  • 05:07Digestion with Proteinase K and Nucleotide Separation
  • 06:12Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
  • 07:50Conclusion

Summary

자동 번역

Deze gewijzigde extractie protocol verbetert RNA en DNA opbrengst van meer doelgerichte regio's van belang in histopathologische weefsel blokken.

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