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Chapters
- 00:05Title
- 01:06Whole Mount Staining
- 03:00Scar Tissue Clearing Method
- 04:27CUBIC Tissue Clearing Method
- 05:42Results: Imaging the Cleared Embryonic Heart
- 07:02Conclusion
We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.
