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在成像钙<em>果蝇</em>在蛋激活

JoVE 신문
발생학

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JoVE 신문 발생학

Imaging Calcium in Drosophila at Egg Activation

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在成像钙<em>果蝇</em>在蛋激活

DOI:

10.3791/54311-v

•

07:45 min

•

August 06, 2016

•

Christopher J. Derrick*¹, Anna H. York-Andersen*¹, Timothy T. Weil

¹Department of Zoology,University of Cambridge

Chapters

00:05Title
00:51Isolating Individual Mature Eggs
02:17Preparing for Imaging
03:28Imaging Ex Vivo Egg Activation
04:43Post-acquisition Image Processing
05:43Results: A Single Ca²⁺ Wave and the Co-visualization of Actin and Ca²⁺ at Drosophila Egg Activation
07:00Conclusion

Summary

자동 번역

本文介绍了激活卵子在果蝇中可视化钙^离子的适应体外协议。

Tags

Keywords: Calcium Imaging Drosophila Egg Activation 발생학 Mature Eggs Ovary Activation Buffer Coverslip Dissecting Microscope Forceps Dissecting Probe Oil GFP Bright Field Z-stack Time Series

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