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Chapters
- 00:05Title
- 01:03Collection and Fertilization of Ciona intestinalis Gametes
- 02:43Dechorionization of Zygotes
- 04:44Electroporation of Zygotes
- 05:58Microinjection of Unfertilized Dechorionated Eggs
- 07:40Results: Microinjection and Electroporation of Ciona intestinalis Embryos
- 08:51Conclusion
We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.
