Journal
/
/
Een werkwijze van gerichte Cell Isolation via Glasoppervlak functionalisering
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
A Method of Targeted Cell Isolation via Glass Surface Functionalization
DOI:

10:40 min

September 20, 2016

, , , ,

Chapters

  • 00:05Title
  • 01:03APTES and DSB Functionalization
  • 03:58Streptavidin Functionalization
  • 04:58Cell Capture and Release
  • 07:36Results: Functionalized Cell Capture of MCF7GFP and RAW Macrophage Cells
  • 09:18Conclusion

Summary

자동 번역

This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

Related Videos

Read Article