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Driedimensionale Culture Assay te ontdekken Cancer Cell Invasiviteit en Satellite tumorvorming
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JoVE 신문 의학
Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation
DOI:

09:23 min

August 18, 2016

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Chapters

  • 00:05Title
  • 01:00Pseudo-primary Tumor Collagen Plug Preparation
  • 02:26First Fibrin Gel Layer Preparation and Fibrin Gel Generation
  • 04:03Second Fibrin Gel Layer Preparation and Collagen Plug Sandwiching
  • 05:33Cell Culture Medium Conditions
  • 06:27Results: Representative Tumor Cell 3D Culture, Invasiveness, and Satellite Tumor Formation
  • 07:58Conclusion

Summary

자동 번역

Cancer cells are embedded in a collagen gel and then sandwiched in an acellular fibrin gel to generate a 3D culture system in which the invasiveness and formation of satellite tumors may be monitored.

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