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Chapters
- 00:05Title
- 00:58Fabrication of Polystyrene Microbead Molds
- 02:48Formation of Bare and ECM-coated PEGDA Scaffolds
- 06:39Huh-7.5 Cell Culture and Seeding
- 08:17Results: An Effective 3D Cell Culture Platform for Liver Tissue Engineering
- 09:23Conclusion
This manuscript presents a detailed protocol for the fabrication of an emerging three-dimensional hepatocyte culture platform, the inverted colloidal crystal scaffold, and the concomitant techniques to assess hepatocyte behavior. The size-controllable pores, interconnectivity and ability to conjugate extracellular matrix proteins to the poly(ethylene glycol) (PEG) scaffold enhance Huh-7.5 cell performance.
