Journal
/
유전학
/
Genetic Engineering af en utraditionel Gær for Vedvarende Biobrændsel og biokemisk Produktion
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering af en utraditionel Gær for Vedvarende Biobrændsel og biokemisk Produktion

DOI:
10.3791/54371-v

•

10:10 min

•

September 20, 2016

•

, , , , , ,
1Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, 2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute,National University of Singapore, 3Food Science and Chemical Engineering,Singapore Institute of Technology

Chapters

  • 00:05Title
  • 01:18Construction of the Disruption Cassette for Deleting KU70
  • 03:08Generation of the Y. lipolytica KU70 Deletion Strain
  • 05:45Cre Recombinase-mediated Marker Rescue
  • 07:59Results: Deletion of KU70 Enhances Gene Deletion Efficiency
  • 09:27Conclusion

Summary

자동 번역

자동 번역

We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.

Tags

Genetic EngineeringUnconventional YeastRenewable BiofuelBiochemical ProductionYarrowia LipolyticaPO1G StrainGene DeletionHomologous RecombinationTransformation ProtocolLoxP SitesLEU2 Expression CassetteKU70 GeneDisruption Cassette

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/54371?article_type=v

En robot platform for High-throughput protoplastisolering og Transformation
check_url/kr/54371?article_type=v

En universel protokol for storstilet gRNA bibliotek produktion fra enhver kilde, DNA
check_url/kr/54371?article_type=v

Produktion og oprensning af Baculovirus for genterapi ansøgning
check_url/kr/54371?article_type=v

Høj opløsning sammenligning af bakteriel konjugation frekvenser
check_url/kr/54371?article_type=v

Isolation, karakterisering og mikroRNA-baseret genetisk modificering af menneskelige Dental follikel stamceller
check_url/kr/54371?article_type=v

Gensplejsning af Dictyostelium discoideum celler baseret på udvælgelse og vækst på bakterier
check_url/kr/54371?article_type=v

En dyb-sekventering-assisteret, spontane Suppressor skærm i Fission gær Schizosaccharomyces pombe
check_url/kr/54371?article_type=v

Halvleder sekvensering for præimplantations genetisk testning for Aneuploidy
check_url/kr/54371?article_type=v

Samlede CRISPR-baserede genetiske skærme i pattedyrsceller
check_url/kr/54371?article_type=v

Genetisk kortlægning af termo Toleranceforskelle mellem arter af Saccharomyces gær via Genome-Wide gensidig Hemizygosity analyse

Read Article