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JoVE Journal Genetics
Flowsortering og Exome-sekventering af Reed-Sternberg-cellerne af klassisk Hodgkin-lymfom
 
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Flowsortering og Exome-sekventering af Reed-Sternberg-cellerne af klassisk Hodgkin-lymfom

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Her beskriver vi en kombineret flowcytometrisk celle sortering og lav input, næste generations bibliotek konstruktion protokol designet til at producere højkvalitets, hel-exome data fra Hodgkin Reed-Sternberg (HRS) celler af klassisk Hodgkin lymfom (CHL).

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Genetik udgave 124 klassisk Hodgkin lymfom cHL Reed-Sternberg FACS exome næste generations sekventeringsbibliotek flowcellesortering
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