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JoVE Journal Genetics
Flow-sortering en Exome Sequencing van de Reed-Sternberg Cellen van Klassieke Hodgkin Lymfoom
 
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Flow-sortering en Exome Sequencing van de Reed-Sternberg Cellen van Klassieke Hodgkin Lymfoom

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Hier beschrijven we een gecombineerde flow cytometrische celsortering en een laag ingevoerde, volgende generatie bibliotheekconstructieprotocol dat is ontworpen om hoogwaardige, volledige exome data van de Hodgkin Reed-Sternberg (HRS) cellen van klassiek Hodgkin lymfoom (CHL) te produceren.

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Genetica nummer 124 klassieke Hodgkin lymfoom cHL Reed-Sternberg FACS exome sequentie bibliotheek van de volgende generatie flow cell-sorting
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