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JoVE Journal Genetics
Le tri de flux et le séquençage Exome des cellules de Reed-Sternberg du lymphome de Hodgkin classique
 
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Le tri de flux et le séquençage Exome des cellules de Reed-Sternberg du lymphome de Hodgkin classique

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Ici, nous décrivons un protocole de calcul de la cellule de cytométrie à flux combiné et un protocole de construction de bibliothèque de prochaine génération conçu pour produire des données de haute qualité et exhaustives des cellules Hodgkin Reed-Sternberg (HRS) du lymphome de Hodgkin classique (CHL).

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Genetics lymphome classique de Hodgkin cHL Reed-Sternberg FACS exome bibliothèque de séquençage de la prochaine génération triage des cellules d'écoulement
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