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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

DOI:
10.3791/54402-v

•

09:34 min

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September 20, 2016

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1National Research Institute of Police Science

Chapters

  • 00:05Title
  • 00:45DNA Extraction
  • 02:58PCR and Enzymatic Digestion
  • 04:32Liquid Chromatography/Electrospray Ionization Mass Spectrometry (LC/ESI-MS) Analysis
  • 07:30Results: Representative Results of the Analysis of Raw Muscle from Takifugu rubripes
  • 08:44Conclusion

Summary

자동 번역

자동 번역

An improved polymerase chain reaction-restriction fragment length polymorphism method for genotyping pufferfish species by liquid chromatography/mass spectrometry is described. A reverse-phase silica monolith column is employed for separating digested amplicons. This method can elucidate the monoisotopic masses of oligonucleotides, which is useful for identifying base composition.

Tags

Keywords: Puffer Fish GenotypingToxic Puffer FishPCR-RFLPLiquid Chromatography/mass SpectrometryDNA ExtractionUV SpectrophotometryAnalytical ColumnReagent Contamination

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