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Chapters
- 00:05Title
- 01:24Microgel Synthesis: Conventional Batch Synthesis of Poly(N-isopropylacrylamide) (PNIPAM) Matrix Microgels
- 03:14Microgel Synthesis: Non-stirred Synthesis of Fluorecently Labled Poly(N-isopropylacrylamide) (PNIPAM) Microgels
- 05:40Light Scattering Characterization
- 07:00Particle Tracking by Wide-field Fluorescence Microscopy
- 08:51Results: Dynamic Light Scattering Characterization and Fluorescence Tracking of Microgels in Concentrated Dispersions
- 10:29Conclusion
Non-stirred precipitation polymerization provides a rapid, reproducible prototyping approach to the synthesis of stimuli-sensitive poly(N-isopropylacrylamide) microgels of narrow size distribution. In this protocol synthesis, light scattering characterization and single particle fluorescence tracking of these microgels in a wide-field microscopy setup are demonstrated.
