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Chapters
- 00:05Title
- 01:09Resuscitiation of Frozen HEI-OC1 Cells
- 02:38Subculture of HEI-OC1 Cells
- 04:14Drug Cytotoxicity Studied with HEI-OC1 Cells
- 04:57Electrophysiology Experiments with HEI-OC1 Cells
- 06:14Results: Viability, Death, and Prestin Expression in HEI-OC1 Cells and NP-HEI-OC1 Cells and NLC Studies
- 08:19Conclusion
House Ear Institute-Organ of Corti 1 (HEI-OC1) is one of the few mouse auditory cell lines currently available for research purposes. This protocol describes how to work with HEI-OC1 cells to investigate the cytotoxic effects of pharmacological drugs as well as functional properties of inner ear proteins.
