/

/

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

JoVE 신문
생화학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생화학

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

DOI:

10.3791/54488-v

•

10:39 min

•

November 28, 2016

•

Danielle L. Michell*¹, Ryan M. Allen*¹, Stuart R. Landstreet, Shilin Zhao, Cynthia L. Toth, Quanhu Sheng, Kasey C. Vickers²

¹Department of Medicine,Vanderbilt University School of Medicine, ²Center for Quantitative Sciences,Vanderbilt University School of Medicine, ³Department of Cancer Biology,Vanderbilt University School of Medicine

Chapters

00:05Title
01:13Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 1
03:07Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 2
04:31Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 3
06:05Fast-protein Liquid Chromatography (FPLC) for HDL Purification
08:05Results: Importance of DGUC to Procedure and Small RNA Library Analysis
09:24Conclusion

Summary

자동 번역

This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.

Tags

Keywords: HDL Lipoprotein Small RNA Non-coding RNA Density Gradient Ultracentrifugation Biomarker Cardiometabolic Disease High-throughput Sequencing Real-time PCR

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Read Article